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Solution selection and preparation The solution used to suspend the crude NPC suspension and to dilute the OptiPrep™ may be a routine buffered saline such as PBS [4,5], which may be supplemented with 1% BSA [6] or a balanced salt solution such as Hank’s Balanced Salt Solution (HBSS) [7-11] or an NPC customized medium such as Gey’s balanced salt solution [12-15] and this may be prepared as described in the box

Red Blood Cell Production

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