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Then cells were washed three times with PBS followed by incubation with the secondary antibody (1:200, Proteintech) and Hoechst 33342 (1:1000) for 1 h

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Precisely, superoxide anion and hydrogen peroxide have been reported to act as mitogens, thereby promoting the rate of DNA replication and cell proliferation in a variety of cultured cells.9, 10 Nonetheless, in excess amounts, oxygen-derived radicals are very harmful to living organisms