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On bGLP-1 template, alanine scanning studies from position 10 (to ensure a conservative N-terminal sequence required for GLP-1R activation) afforded novel derivatives ( 6 (EC 50 value of 0.018 nM in GLP-1R activation assay, Figure 10) derived from Ala2 substitution displayed enhanced potency in activating GLP-1R, and was selected for further modifications
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