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The supernatant after centrifugation was loaded onto a Ni-NTA column in the buffer containing 20 mM MOPS, pH 7.4, 100 mM NaCl, and 0.1% (w/v) digitonin, and the eluted fraction including the protein of interest was loaded onto a Superdex 200 (GE Healthcare) column equilibrated in a buffer containing 20 mM MOPS, pH 7.4, 100 mM NaCl and 0.1% (w/v) digitonin

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In the research of Zhou et al, 143 porous scaffolds of PVA were prepared by quenching in liquid nitrogen and the freeze drying method, from different concentration aqueous solutions loaded with ciprofloxacin were fabricated