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Change of inoculation route In addition to sequential immunization (mixed-vaccination), development of new vaccines (such as nanoparticle vaccine), and adjuvant improvement, changing the vaccination route is also a feasible measure to improve the protection and efficacy of existing COVID-19 vaccines
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Cells were labeled with 2 μM fluorescent nitric oxide indicator DFA-FM DA and fluorescence was detected by fluorescent microscope with a standard fluorescein bandpass filter (FITC, 500–555 nm)
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