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Synthesis and vesicular loading of spermidine and spermine co-transmitters The polyamines putrescine (Put), spermidine (Spd), and spermine (Spm) are ubiquitously synthesized across the kingdoms of life, including in bacteria, plants, fungi, and animals (Miller-Fleming et al., 2015)

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IVM medium applied in this study was TCM-199 medium supplemented with 0.3 mM sodium pyruvate, 1 μg/ml β-2-oestradiol, 2 mM Gluta MAX , 10 ng/ml EGF, 10% fetal bovine serum, 10 U/ml FSH, 10 U/ml LH and 100 μM cysteamine ( In vitro fertilization and embryonic development analyses COCs after IVM were collected and transferred to HEPES-synthetic oviductal fluid (H-SOF) medium supplemented with 400 IU/ml hyaluronidase