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Although these direct antioxidant effects are observable in vitro, their significance in vivo appears limited, stemming from low plasma concentrations of NAC and the prevalence of more abundant endogenous antioxidants such as GSH, ascorbate, and antioxidant enzymes (e.g., SODs, GPs, CAT) [6,38,39]

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The Johns Hopkins University animal facility is accredited by the American Association for the Accreditation of Laboratory Animal Care and meets National Institute of Health standards as set forth in the “Guide for the Care and Use of Laboratory Animals” (DHHS Publication No