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After washing with TBST (50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween-20 and pH 7.6), the fixed cells were blocked using Blocking One reagent (Nacalai Tesque) containing 0.1% Triton X-100 for 1 h at 25 °C

Inflammation

Size:1000 mcg/ml - 30 ml (Pack of 5)

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